Histology and Microscopic Image Analyses - Short Profile

Histology / morphometry:

Conventional and immunohistochemical staining, especially hypothalamic and placental serial sections (paraffin and frozen sections), followed by stereology and computerized morphometry using microscopic image analysis:

Example:

Opens external link in current windowProgramming' of orexigenic and anorexigenic hypothalamic neurons in offspring of treated and untreated diabetic mother rats.

Parameters:
Topographic - volumes, area percentages, total cell count, fetal-maternal exchange surface (placenta)

Cellular - numerical density, optical density, nuclear size, cell and cytoplasm size, nuclear-cytoplasmic ratio

Marker:
Nissle +, GFAP +, NPY +, CCK-8S +, galanin +, AGRP + CRF +, TH +, POMC +, alpha-MSH +, C4-Res +, leptin-Res +, Insulin-Res +, HPL +, GR +, GRalpha +, caspase 3 +, vWF +.

Placental stereology (cell counting, Feto-maternal exchange area, volume).

 

 

 

Responsible for Histology and Microscopic Image Analyses

Dr. med. Thorsten Braun

Dipl. vet. med. Kerstin Melchior

Loreen Ehrlich

Research database

For more details please use the Charité undefinedresearch database.